General Information
SPKB ID SPKB07020
Signal sequence MKKVVNSVLASALALTVAPMAFA
Length 23
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..23
Activity Information
Pathway Tat
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Alkaline protease yield: 1299.62(U/mL)
Type Tat/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07020.cif
Physicochemical Information
Molecular weight 2332.88864
Aliphatic index 127.3913043
Isoelectric point 10.80537956
Charge (pH=7) 1.997394232
Topological polar surface area 850.55
Aromaticity 0.043478261
Average rotatable bonds 3.260869565
Number of hydrogen bond donors 29
Number of hydrogen bond acceptors 32
X logP energy -6.3005
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
