General Information
SPKB ID SPKB07019
Signal sequence MKIRKILLSSALSFGMLISAVPALA
Length 25
Organism Bacillus subtilis WB600
UniProt ID O31836,
Motif 1..25
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 1490.736(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07019.cif
Physicochemical Information
Molecular weight 2631.31714
Aliphatic index 152.4
Isoelectric point 11.82305527
Charge (pH=7) 2.99739092
Topological polar surface area 947.79
Aromaticity 0.04
Average rotatable bonds 3.56
Number of hydrogen bond donors 34
Number of hydrogen bond acceptors 35
X logP energy -5.48513
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
