SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07016

Signal sequence   MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAA

Length  46

Organism  Bacillus subtilis WB600

UniProt ID  NA,

Motif  1..46



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline protease yield: 1815.64(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07016.cif

Structure  SPKB07016.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4954.90104

Aliphatic index  91.52173913

Isoelectric point  11.65059825

Charge (pH=7)   4.0915486

Topological polar surface area  1996.69

Aromaticity  0.043478261

Average rotatable bonds  3.673913043

Number of hydrogen bond donors  73

Number of hydrogen bond acceptors  72

X logP energy  -21.35929

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.