General Information
SPKB ID SPKB07015
Signal sequence MKKGIIRFLLVSFVLFFALSTGITGVQAA
Length 29
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..29
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 2369.89(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07015.cif
Physicochemical Information
Molecular weight 3128.85294
Aliphatic index 134.4827586
Isoelectric point 11.82305527
Charge (pH=7) 2.99739092
Topological polar surface area 1116.07
Aromaticity 0.137931034
Average rotatable bonds 3.586206897
Number of hydrogen bond donors 40
Number of hydrogen bond acceptors 39
X logP energy -5.52583
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
