General Information
SPKB ID SPKB07013
Signal sequence MKLKSKLFVICLAAAAIFTAAGVSANA
Length 27
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 3506.42(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07013.cif
Physicochemical Information
Molecular weight 2710.33194
Aliphatic index 123.3333333
Isoelectric point 10.51301932
Charge (pH=7) 2.935124482
Topological polar surface area 1001.76
Aromaticity 0.074074074
Average rotatable bonds 3.333333333
Number of hydrogen bond donors 36
Number of hydrogen bond acceptors 37
X logP energy -7.8677
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
