General Information
SPKB ID SPKB07012
Signal sequence MVSIRRSFEAYVDDMNIITVLIPAEQKEI
Length 29
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..29
Activity Information
Pathway Tat
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Alkaline protease yield: 3631.28(U/mL)
Type Tat/SPI
Structure Information
PDB ID 2E8Y,
Predicted Structure ID SPKB07012.cif
Physicochemical Information
Molecular weight 3381.95034
Aliphatic index 117.5862069
Isoelectric point 4.264691876
Charge (pH=7) -1.996949435
Topological polar surface area 1372.75
Aromaticity 0.068965517
Average rotatable bonds 3.931034483
Number of hydrogen bond donors 47
Number of hydrogen bond acceptors 46
X logP energy -9.83386
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
