General Information
SPKB ID SPKB07010
Signal sequence MKVCQKSIVRFLVSLIIGTFVISVPFMANA
Length 30
Organism Bacillus subtilis WB600
UniProt ID O35015,
Motif 1..30
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 4755.07(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07010.cif
Physicochemical Information
Molecular weight 3313.12454
Aliphatic index 133
Isoelectric point 10.79319833
Charge (pH=7) 2.935416204
Topological polar surface area 1179.47
Aromaticity 0.1
Average rotatable bonds 3.633333333
Number of hydrogen bond donors 42
Number of hydrogen bond acceptors 43
X logP energy -6.62453
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
