General Information
SPKB ID SPKB07009
Signal sequence MRMKRLRMRKHLLIAVCTLALLLSSPIVSDA
Length 31
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..31
Activity Information
Pathway Tat
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Alkaline protease yield: 5160.24(U/mL)
Type Tat/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07009.cif
Physicochemical Information
Molecular weight 3537.45234
Aliphatic index 141.6129032
Isoelectric point 12.07925983
Charge (pH=7) 5.026761846
Topological polar surface area 1374.07
Aromaticity 0
Average rotatable bonds 3.935483871
Number of hydrogen bond donors 52
Number of hydrogen bond acceptors 48
X logP energy -9.46652
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
