General Information
SPKB ID SPKB07007
Signal sequence MKKRLIQVMIMFTLLLTMAFSADAA
Length 25
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..25
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 5606.19(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07007.cif
Physicochemical Information
Molecular weight 2844.58644
Aliphatic index 121.2
Isoelectric point 10.78900924
Charge (pH=7) 1.997837405
Topological polar surface area 1016.74
Aromaticity 0.08
Average rotatable bonds 3.92
Number of hydrogen bond donors 36
Number of hydrogen bond acceptors 38
X logP energy -4.44113
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
