General Information
SPKB ID SPKB07006
Signal sequence MKKMLMLAFTFLLALTIHVGEASAV
Length 25
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..25
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 5962.94(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07006.cif
Physicochemical Information
Molecular weight 2736.42814
Aliphatic index 132.8
Isoelectric point 9.539374794
Charge (pH=7) 1.090078445
Topological polar surface area 940.43
Aromaticity 0.08
Average rotatable bonds 3.68
Number of hydrogen bond donors 33
Number of hydrogen bond acceptors 36
X logP energy -3.0797
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
