SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07005

Signal sequence   MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAE

Length  34

Organism  Bacillus subtilis WB600

UniProt ID  NA,

Motif  1..34



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline protease yield: 6001.168(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07005.cif

Structure  SPKB07005.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3649.44124

Aliphatic index  120.8823529

Isoelectric point  10.78905182

Charge (pH=7)   2.090075134

Topological polar surface area  1246.65

Aromaticity  0.147058824

Average rotatable bonds  3.382352941

Number of hydrogen bond donors  43

Number of hydrogen bond acceptors  44

X logP energy  -4.48013

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.