General Information
SPKB ID SPKB07004
Signal sequence MFKFVTKVLTVVIAATISFCLSAVPASAN
Length 29
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..29
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 6115.84(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07004.cif
Physicochemical Information
Molecular weight 3029.68964
Aliphatic index 121.0344828
Isoelectric point 9.676237541
Charge (pH=7) 1.935419516
Topological polar surface area 1085.84
Aromaticity 0.103448276
Average rotatable bonds 3.275862069
Number of hydrogen bond donors 39
Number of hydrogen bond acceptors 41
X logP energy -8.2935
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
