General Information
SPKB ID SPKB07002
Signal sequence LKRMIVRMTLPLLIVCLAFSSFSASARAA
Length 29
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..29
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 6268.74(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07002.cif
Physicochemical Information
Molecular weight 3166.93864
Aliphatic index 131.3793103
Isoelectric point 12.22275045
Charge (pH=7) 3.935704616
Topological polar surface area 1182.2
Aromaticity 0.068965517
Average rotatable bonds 3.586206897
Number of hydrogen bond donors 45
Number of hydrogen bond acceptors 42
X logP energy -8.59669
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
