General Information
SPKB ID SPKB07001
Signal sequence MRKKTKNRLISSVLSTVVISSLLFPGAAGAS
Length 31
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..31
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 6708.31(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07001.cif
Physicochemical Information
Molecular weight 3232.87604
Aliphatic index 113.2258065
Isoelectric point 12.54607567
Charge (pH=7) 4.997092575
Topological polar surface area 1334.32
Aromaticity 0.032258065
Average rotatable bonds 3.548387097
Number of hydrogen bond donors 49
Number of hydrogen bond acceptors 47
X logP energy -15.48426
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
