General Information
SPKB ID SPKB07000
Signal sequence MKRLFMKASLVLFAVVFVFAVKGAPAKA
Length 28
Organism Bacillus subtilis WB600
UniProt ID O34554,
Motif 1..28
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 6790.49(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07000.cif
Physicochemical Information
Molecular weight 3040.85154
Aliphatic index 115
Isoelectric point 11.99738138
Charge (pH=7) 4.996800853
Topological polar surface area 1026.44
Aromaticity 0.142857143
Average rotatable bonds 3.571428571
Number of hydrogen bond donors 36
Number of hydrogen bond acceptors 37
X logP energy -2.58823
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
