General Information
SPKB ID SPKB06999
Signal sequence MMKKLFHSTLIVLLFFSFFGVQPIHAK
Length 27
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 6969.5(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06999.cif
Physicochemical Information
Molecular weight 3180.94914
Aliphatic index 111.8518519
Isoelectric point 11.10310314
Charge (pH=7) 3.17891738
Topological polar surface area 1050.33
Aromaticity 0.185185185
Average rotatable bonds 3.851851852
Number of hydrogen bond donors 36
Number of hydrogen bond acceptors 39
X logP energy -1.5816
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
