SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06996

Signal sequence   MSDEQKKPEQIHRRDILKWGAMAGAAVAIGASGLGGLAPLVQTAAKP

Length  47

Organism  Bacillus subtilis WB600

UniProt ID  NA,

Motif  1..47



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  alpha-amylase yield: 9.1(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  7PKX,

Predicted Structure ID  SPKB06996.cif

Structure  SPKB06996.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4840.63964

Aliphatic index  89.57446809

Isoelectric point  10.32593878

Charge (pH=7)   2.092149846

Topological polar surface area  1987.06

Aromaticity  0.021276596

Average rotatable bonds  3.446808511

Number of hydrogen bond donors  67

Number of hydrogen bond acceptors  67

X logP energy  -20.10066

Hydropathy 



Literature Information


Title  Enhanced extracellular production of L-asparaginase from Bacillus subtilis 168 by B-subtilis WB600 through a combined strategy

DOI  10.1007/s00253-016-7816-x,

Publication Year  2017

PubMed ID  27796436,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.