SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06994

Signal sequence   MKFVKRRIIALVTILMLSVTSLFALQPSAKA

Length  31

Organism  Bacillus subtilis WB600

UniProt ID  P37957,

Motif  1..31



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  alpha-amylase yield: 18.2(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06994.cif

Structure  SPKB06994.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3447.33134

Aliphatic index  141.6129032

Isoelectric point  12.54607567

Charge (pH=7)   4.997092575

Topological polar surface area  1273.63

Aromaticity  0.064516129

Average rotatable bonds  3.774193548

Number of hydrogen bond donors  46

Number of hydrogen bond acceptors  45

X logP energy  -7.22596

Hydropathy 



Literature Information


Title  Enhanced extracellular production of L-asparaginase from Bacillus subtilis 168 by B-subtilis WB600 through a combined strategy

DOI  10.1007/s00253-016-7816-x,

Publication Year  2017

PubMed ID  27796436,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.