SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06992

Signal sequence   MKKRKRRNFKRFIAAFLVLALMISLVPADVLA

Length  32

Organism  Bacillus subtilis WB600

UniProt ID  H9TZU6,

Motif  1..32



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  alpha-amylase yield: 28.9(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06992.cif

Structure  SPKB06992.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3717.67154

Aliphatic index  128.125

Isoelectric point  12.53169954

Charge (pH=7)   6.997237403

Topological polar surface area  1408.93

Aromaticity  0.09375

Average rotatable bonds  4

Number of hydrogen bond donors  51

Number of hydrogen bond acceptors  46

X logP energy  -6.53072

Hydropathy 



Literature Information


Title  Enhanced extracellular production of L-asparaginase from Bacillus subtilis 168 by B-subtilis WB600 through a combined strategy

DOI  10.1007/s00253-016-7816-x,

Publication Year  2017

PubMed ID  27796436,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.