SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06991

Signal sequence   MSDEQKKPEQIHRRDILKWGAMAGAAVA

Length  28

Organism  Bacillus subtilis WB600

UniProt ID  P39597,

Motif  1..28



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  L-Asparaginase yield: 32.42(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  7PKX,

Predicted Structure ID  SPKB06991.cif

Structure  SPKB06991.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3137.62514

Aliphatic index  70

Isoelectric point  9.44043693

Charge (pH=7)   1.09244488

Topological polar surface area  1342.17

Aromaticity  0.035714286

Average rotatable bonds  3.857142857

Number of hydrogen bond donors  46

Number of hydrogen bond acceptors  44

X logP energy  -12.53746

Hydropathy 



Literature Information


Title  Study on the expression and fermentation optimization of L-asparaginase in Bacillus subtilis WB600

DOI  NA,

Publication Year  2015

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.