SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06955

Signal sequence   MKKRKRRNFKRFIAAFLVLALLMISLVPADVLAKST

Length  36

Organism  Bacillus subtilis WB700

UniProt ID  NA,

Motif  1..36



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Alkaline active xylanase yield: 10.3(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06955.cif

Structure  SPKB06955.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4147.18834

Aliphatic index  124.7222222

Isoelectric point  12.53897435

Charge (pH=7)   7.996942369

Topological polar surface area  1591.81

Aromaticity  0.083333333

Average rotatable bonds  4

Number of hydrogen bond donors  58

Number of hydrogen bond acceptors  53

X logP energy  -9.04332

Hydropathy 



Literature Information


Title  Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening

DOI  10.1007/s00253-016-7615-4,

Publication Year  2016

PubMed ID  27225471,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.