SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06953

Signal sequence   MARKKKKKHEDEHVDESWLVPYADILTLLLALFIVLYASS

Length  40

Organism  Bacillus subtilis WB700

UniProt ID  NA,

Motif  1..40



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline active xylanase yield: 13.8(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  6YSL,

Predicted Structure ID  SPKB06953.cif

Structure  SPKB06953.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4672.50614

Aliphatic index  119.5

Isoelectric point  7.692794607

Charge (pH=7)   0.183287993

Topological polar surface area  1799.76

Aromaticity  0.1

Average rotatable bonds  3.925

Number of hydrogen bond donors  63

Number of hydrogen bond acceptors  62

X logP energy  -8.89333

Hydropathy 



Literature Information


Title  Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening

DOI  10.1007/s00253-016-7615-4,

Publication Year  2016

PubMed ID  27225471,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.