SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06943

Signal sequence   MKKELLASLVLCLSLSPLVSTNEVFA

Length  26

Organism  Bacillus subtilis WB700

UniProt ID  O31635,

Motif  1..26



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline active xylanase yield: 35.8(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  9A4P,

Predicted Structure ID  SPKB06943.cif

Structure  SPKB06943.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2806.41204

Aliphatic index  146.1538462

Isoelectric point  6.354537022

Charge (pH=7)   -0.061030239

Topological polar surface area  1052.91

Aromaticity  0.038461538

Average rotatable bonds  3.615384615

Number of hydrogen bond donors  37

Number of hydrogen bond acceptors  39

X logP energy  -7.5064

Hydropathy 



Literature Information


Title  Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening

DOI  10.1007/s00253-016-7615-4,

Publication Year  2016

PubMed ID  27225471,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.