SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06902

Signal sequence   MLKKKWMVGLLAGCLAAGGFSYNAFA

Length  26

Organism  Bacillus subtilis WB700

UniProt ID  O35012,

Motif  1..26



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline active xylanase yield: 112(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06902.cif

Structure  SPKB06902.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2748.35774

Aliphatic index  90.38461538

Isoelectric point  10.13347695

Charge (pH=7)   2.934274068

Topological polar surface area  968.22

Aromaticity  0.153846154

Average rotatable bonds  3.461538462

Number of hydrogen bond donors  35

Number of hydrogen bond acceptors  36

X logP energy  -5.1678

Hydropathy 



Literature Information


Title  Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening

DOI  10.1007/s00253-016-7615-4,

Publication Year  2016

PubMed ID  27225471,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.