General Information
SPKB ID SPKB06898
Signal sequence MKKLLAAGIIGLLTVSIASPSFA
Length 23
Organism Bacillus subtilis WB700
UniProt ID P70961,
Motif 1..23
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline active xylanase yield: 114.5(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06898.cif
Physicochemical Information
Molecular weight 2301.85644
Aliphatic index 148.6956522
Isoelectric point 10.80537956
Charge (pH=7) 1.997394232
Topological polar surface area 827.69
Aromaticity 0.043478261
Average rotatable bonds 3.304347826
Number of hydrogen bond donors 29
Number of hydrogen bond acceptors 31
X logP energy -5.4973
Hydropathy 
Literature Information
Title Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening
DOI 10.1007/s00253-016-7615-4,
Publication Year 2016
PubMed ID 27225471,
Patent ID NA,
