SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06885

Signal sequence   MNKKYFVLIVCIIFTSALFPTFSSVTA

Length  27

Organism  Bacillus subtilis WB700

UniProt ID  O32041,

Motif  1..27



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline active xylanase yield: 144.5(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  9A7D,

Predicted Structure ID  SPKB06885.cif

Structure  SPKB06885.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3041.70004

Aliphatic index  111.8518519

Isoelectric point  9.405774727

Charge (pH=7)   1.934569102

Topological polar surface area  1047.87

Aromaticity  0.185185185

Average rotatable bonds  3.518518519

Number of hydrogen bond donors  38

Number of hydrogen bond acceptors  40

X logP energy  -5.3977

Hydropathy 



Literature Information


Title  Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening

DOI  10.1007/s00253-016-7615-4,

Publication Year  2016

PubMed ID  27225471,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.