SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06863

Signal sequence   MKKKKTWKRFLHFSSAALAAGLIFTSAAPAEA

Length  32

Organism  Bacillus subtilis WB700

UniProt ID  P94522,

Motif  1..32



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline active xylanase yield: 222.8(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06863.cif

Structure  SPKB06863.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3479.14684

Aliphatic index  73.75

Isoelectric point  11.36666267

Charge (pH=7)   5.089190032

Topological polar surface area  1331.55

Aromaticity  0.125

Average rotatable bonds  3.59375

Number of hydrogen bond donors  48

Number of hydrogen bond acceptors  47

X logP energy  -10.40913

Hydropathy 



Literature Information


Title  Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening

DOI  10.1007/s00253-016-7615-4,

Publication Year  2016

PubMed ID  27225471,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.