SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06862

Signal sequence   MKLKTKASIKFGICVGLLCLSITGFTPFFNSTHAEA

Length  36

Organism  Bacillus subtilis WB700

UniProt ID  P39824,

Motif  1..36



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline active xylanase yield: 225.7(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06862.cif

Structure  SPKB06862.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3875.66084

Aliphatic index  92.22222222

Isoelectric point  9.601448848

Charge (pH=7)   2.965538946

Topological polar surface area  1427.79

Aromaticity  0.111111111

Average rotatable bonds  3.555555556

Number of hydrogen bond donors  52

Number of hydrogen bond acceptors  54

X logP energy  -11.8893

Hydropathy 



Literature Information


Title  Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening

DOI  10.1007/s00253-016-7615-4,

Publication Year  2016

PubMed ID  27225471,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.