SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06811

Signal sequence   MKMSEKRHRVSRRQFLNYTLTGVGGFMAAGMLMPMVRFA

Length  39

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..39



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Nattokinase yield: 2.28(FU/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06811.cif

Structure  SPKB06811.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4507.45674

Aliphatic index  60

Isoelectric point  12.34593197

Charge (pH=7)   6.089211475

Topological polar surface area  1775.18

Aromaticity  0.102564103

Average rotatable bonds  3.923076923

Number of hydrogen bond donors  65

Number of hydrogen bond acceptors  62

X logP energy  -15.49445

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.