General Information
SPKB ID SPKB06804
Signal sequence MIKKWAVHLLFSALVLLGLSGGAA
Length 24
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..24
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 3.32(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06804.cif
Physicochemical Information
Molecular weight 2496.09524
Aliphatic index 154.5833333
Isoelectric point 10.80542203
Charge (pH=7) 2.088303323
Topological polar surface area 869.59
Aromaticity 0.083333333
Average rotatable bonds 3.416666667
Number of hydrogen bond donors 31
Number of hydrogen bond acceptors 31
X logP energy -2.9146
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
