SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06803

Signal sequence   MLMSAFVLVLAACQQADPKGSYA

Length  23

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..23



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 3.42(FU/mL)

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06803.cif

Structure  SPKB06803.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2414.87834

Aliphatic index  97.82608696

Isoelectric point  6.157533513

Charge (pH=7)   -0.06468938

Topological polar surface area  904.92

Aromaticity  0.086956522

Average rotatable bonds  3.347826087

Number of hydrogen bond donors  31

Number of hydrogen bond acceptors  34

X logP energy  -7.5024

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.