SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06802

Signal sequence   MNQFRMAVIALVLILMTG

Length  18

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..18



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 3.42(FU/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06802.cif

Structure  SPKB06802.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2021.56844

Aliphatic index  151.6666667

Isoelectric point  10.55000222

Charge (pH=7)   0.997980988

Topological polar surface area  726.33

Aromaticity  0.055555556

Average rotatable bonds  3.777777778

Number of hydrogen bond donors  25

Number of hydrogen bond acceptors  26

X logP energy  -2.85943

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.