SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06801

Signal sequence   MKKTFGIASAFILAAGLTAG

Length  20

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..20



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Cell membrane

Secretion efficiency  Nattokinase yield: 3.56(FU/mL)

Type  Sec/SPII



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06801.cif

Structure  SPKB06801.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  1968.38494

Aliphatic index  103

Isoelectric point  10.80537956

Charge (pH=7)   1.997394232

Topological polar surface area  728.95

Aromaticity  0.1

Average rotatable bonds  3.25

Number of hydrogen bond donors  26

Number of hydrogen bond acceptors  27

X logP energy  -5.962

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.