General Information
SPKB ID SPKB06799
Signal sequence MKWKQTLAIAAALILILAAGCSSKSSS
Length 27
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..27
Activity Information
Pathway Tat
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Nattokinase yield: 3.57(FU/mL)
Type Tat/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06799.cif
Physicochemical Information
Molecular weight 2763.34944
Aliphatic index 123.3333333
Isoelectric point 10.51301932
Charge (pH=7) 2.935124482
Topological polar surface area 1078.24
Aromaticity 0.037037037
Average rotatable bonds 3.481481481
Number of hydrogen bond donors 40
Number of hydrogen bond acceptors 40
X logP energy -10.5217
Hydropathy
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,