SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06799

Signal sequence   MKWKQTLAIAAALILILAAGCSSKSSS

Length  27

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..27



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Nattokinase yield: 3.57(FU/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06799.cif

Structure  SPKB06799.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2763.34944

Aliphatic index  123.3333333

Isoelectric point  10.51301932

Charge (pH=7)   2.935124482

Topological polar surface area  1078.24

Aromaticity  0.037037037

Average rotatable bonds  3.481481481

Number of hydrogen bond donors  40

Number of hydrogen bond acceptors  40

X logP energy  -10.5217

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.