SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06793

Signal sequence   MKNVLAVFVVLIFVLGAFGTSGPAEA

Length  26

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  Q65CX5,

Motif  1..26



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Cell membrane

Secretion efficiency  Nattokinase yield: 3.67(FU/mL)

Type  Sec/SPII



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06793.cif

Structure  SPKB06793.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2651.20254

Aliphatic index  131.1538462

Isoelectric point  6.408795646

Charge (pH=7)   -0.000535612

Topological polar surface area  928.9

Aromaticity  0.115384615

Average rotatable bonds  3.192307692

Number of hydrogen bond donors  31

Number of hydrogen bond acceptors  33

X logP energy  -4.8138

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.