SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06789

Signal sequence   MKKTIMSLAAAAAMSATAFGATASA

Length  25

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..25



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 3.76(FU/mL)

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06789.cif

Structure  SPKB06789.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2373.82644

Aliphatic index  71.2

Isoelectric point  10.80537956

Charge (pH=7)   1.997394232

Topological polar surface area  935.14

Aromaticity  0.04

Average rotatable bonds  3.12

Number of hydrogen bond donors  34

Number of hydrogen bond acceptors  37

X logP energy  -12.1646

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.