SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06787

Signal sequence   MKRFKWFALFAALILLLAA

Length  19

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..19



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 4.01(FU/mL)

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06787.cif

Structure  SPKB06787.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2223.83694

Aliphatic index  149.4736842

Isoelectric point  11.82305527

Charge (pH=7)   2.99739092

Topological polar surface area  716.85

Aromaticity  0.210526316

Average rotatable bonds  3.789473684

Number of hydrogen bond donors  26

Number of hydrogen bond acceptors  24

X logP energy  2.22727

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.