SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06783

Signal sequence   MKRFIKERGLAFFLIAAILLWLKTYA

Length  26

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..26



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 4.23(FU/mL)

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06783.cif

Structure  SPKB06783.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3113.88704

Aliphatic index  135.3846154

Isoelectric point  11.11005093

Charge (pH=7)   3.998017284

Topological polar surface area  1086.23

Aromaticity  0.192307692

Average rotatable bonds  4

Number of hydrogen bond donors  40

Number of hydrogen bond acceptors  36

X logP energy  -0.08926

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.