SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06777

Signal sequence   MSKIKWIITLTICTALAFSLFIFFNKA

Length  27

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..27



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 4.48(FU/mL)

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06777.cif

Structure  SPKB06777.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3121.87564

Aliphatic index  126.6666667

Isoelectric point  10.51301932

Charge (pH=7)   2.935124482

Topological polar surface area  1058.01

Aromaticity  0.185185185

Average rotatable bonds  3.777777778

Number of hydrogen bond donors  39

Number of hydrogen bond acceptors  39

X logP energy  -2.8013

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.