General Information
SPKB ID SPKB06774
Signal sequence MKRFFSVIILGALLLLGTSAPIEA
Length 24
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..24
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 4.66(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06774.cif
Physicochemical Information
Molecular weight 2561.16434
Aliphatic index 154.5833333
Isoelectric point 9.695027686
Charge (pH=7) 0.999461077
Topological polar surface area 909.74
Aromaticity 0.083333333
Average rotatable bonds 3.5
Number of hydrogen bond donors 32
Number of hydrogen bond acceptors 32
X logP energy -3.73683
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
