General Information
SPKB ID SPKB06769
Signal sequence MGTKKKLGLGVASAALGLALVGGGTWA
Length 27
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 4.81(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06769.cif
Physicochemical Information
Molecular weight 2528.05124
Aliphatic index 112.2222222
Isoelectric point 11.10307074
Charge (pH=7) 2.997099198
Topological polar surface area 974.46
Aromaticity 0.037037037
Average rotatable bonds 3.148148148
Number of hydrogen bond donors 35
Number of hydrogen bond acceptors 35
X logP energy -9.3171
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
