General Information
SPKB ID SPKB06768
Signal sequence MKKKVYTFGLASILGTASLFTPFMNNTASA
Length 30
Organism Bacillus licheniformis strain 3F-3
UniProt ID Q65NQ9,
Motif 1..30
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 4.94(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06768.cif
Physicochemical Information
Molecular weight 3210.79704
Aliphatic index 75
Isoelectric point 10.64292272
Charge (pH=7) 2.996248784
Topological polar surface area 1224.51
Aromaticity 0.133333333
Average rotatable bonds 3.466666667
Number of hydrogen bond donors 43
Number of hydrogen bond acceptors 46
X logP energy -12.033
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
