SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06766

Signal sequence   MNKKLLGIMMTIILAIAVLGTAAFFVIKGSA

Length  31

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..31



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 5.25(FU/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06766.cif

Structure  SPKB06766.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3237.11024

Aliphatic index  148.0645161

Isoelectric point  11.10307074

Charge (pH=7)   2.997099198

Topological polar surface area  1118.16

Aromaticity  0.064516129

Average rotatable bonds  3.580645161

Number of hydrogen bond donors  39

Number of hydrogen bond acceptors  42

X logP energy  -4.5761

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.