SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06765

Signal sequence   MKKLISIIFIFVLGVVGSLTAAVSAEA

Length  27

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..27



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 5.27(FU/mL)

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06765.cif

Structure  SPKB06765.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2778.42964

Aliphatic index  158.8888889

Isoelectric point  9.537300927

Charge (pH=7)   0.999169354

Topological polar surface area  990.18

Aromaticity  0.074074074

Average rotatable bonds  3.444444444

Number of hydrogen bond donors  35

Number of hydrogen bond acceptors  36

X logP energy  -4.9888

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.