SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06760

Signal sequence   MKKLSEESLKDNTFDRRRFIQGAGKIAGLSLGLAIAQSMGAMEVNA

Length  46

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..46



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Nattokinase yield: 5.73(FU/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06760.cif

Structure  SPKB06760.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4954.75584

Aliphatic index  87.17391304

Isoelectric point  10.32219448

Charge (pH=7)   2.003012567

Topological polar surface area  2122.61

Aromaticity  0.043478261

Average rotatable bonds  3.847826087

Number of hydrogen bond donors  74

Number of hydrogen bond acceptors  71

X logP energy  -23.25839

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.