General Information
SPKB ID SPKB06759
Signal sequence MKKSLFLFVFSLFLMAIPAFS
Length 21
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..21
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 5.86(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06759.cif
Physicochemical Information
Molecular weight 2438.07014
Aliphatic index 116.1904762
Isoelectric point 10.80537956
Charge (pH=7) 1.997394232
Topological polar surface area 749.26
Aromaticity 0.238095238
Average rotatable bonds 3.714285714
Number of hydrogen bond donors 26
Number of hydrogen bond acceptors 29
X logP energy 0.4814
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
