SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06750

Signal sequence   MKRIYIFLLCFAVLLPVGGKTAQA

Length  24

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..24



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Nattokinase yield: 6.55(FU/mL)

Type  NA



Structure Information


PDB ID  4ZOQ,

Predicted Structure ID  SPKB06750.cif

Structure  SPKB06750.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2653.32584

Aliphatic index  134.1666667

Isoelectric point  10.25587917

Charge (pH=7)   2.93456579

Topological polar surface area  921.32

Aromaticity  0.125

Average rotatable bonds  3.583333333

Number of hydrogen bond donors  33

Number of hydrogen bond acceptors  33

X logP energy  -2.74983

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.