General Information
SPKB ID SPKB06748
Signal sequence MPKKNKFMNRGAAILSICFALFFFVIVGRFA
Length 31
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..31
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 6.64(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06748.cif
Physicochemical Information
Molecular weight 3538.37984
Aliphatic index 94.51612903
Isoelectric point 11.7516056
Charge (pH=7) 4.935117859
Topological polar surface area 1235.8
Aromaticity 0.193548387
Average rotatable bonds 3.741935484
Number of hydrogen bond donors 44
Number of hydrogen bond acceptors 43
X logP energy -5.11776
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
