General Information
SPKB ID SPKB06742
Signal sequence MRKSIVRYFVMAFILLFALSTFLTGVQA
Length 28
Organism Bacillus licheniformis strain 3F-3
UniProt ID NA,
Motif 1..28
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Nattokinase yield: 8.72(FU/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB06742.cif
Physicochemical Information
Molecular weight 3223.97174
Aliphatic index 125.3571429
Isoelectric point 11.53052052
Charge (pH=7) 2.996832229
Topological polar surface area 1143.08
Aromaticity 0.178571429
Average rotatable bonds 3.75
Number of hydrogen bond donors 42
Number of hydrogen bond acceptors 40
X logP energy -4.48386
Hydropathy 
Literature Information
Title High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase
DOI 10.1111/jam.13175,
Publication Year 2016
PubMed ID 27159567,
Patent ID NA,
