SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06739

Signal sequence   MKRLTSVLASAFAVILLFG

Length  19

Organism  Bacillus licheniformis strain 3F-3

UniProt ID  NA,

Motif  1..19



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Cell membrane

Secretion efficiency  Nattokinase yield: 13.11(FU/mL)

Type  Sec/SPII



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06739.cif

Structure  SPKB06739.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2037.53464

Aliphatic index  148.9473684

Isoelectric point  11.65177567

Charge (pH=7)   1.997685954

Topological polar surface area  735.73

Aromaticity  0.105263158

Average rotatable bonds  3.526315789

Number of hydrogen bond donors  27

Number of hydrogen bond acceptors  26

X logP energy  -3.64553

Hydropathy 



Literature Information


Title  High-level expression of nattokinase in Bacillus licheniformis by manipulating signal peptide and signal peptidase

DOI  10.1111/jam.13175,

Publication Year  2016

PubMed ID  27159567,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.